Database : Protein Data Bank ENT file coordinates (NPSA3DB) [more template choice] Protein Data Bank ENT file coordinates clustered at 95+% sequence identity (NPSA3D95) Protein Data Bank ENT file coordinates clustered at 25+% sequence identity (NPSA3D25) [useful for query multi-domain protein]

Sequence 1 name (optional) :
Paste protein 1 sequence below : help


Expectation value (-e, real) : 1e-06 1e-03 1e-02 1e-01 1.0 10.0
Number of template to show (-v, int == -b, int) : 100 250 500 1000 2500

PSI-BLAST PSSM building stage parameters (using non-redundant UniProtKB maximum 50% identity and at most 10,000 alignments):
Filter query sequence (-F) : TrueFalse (DUST with BLASTN, SEG with others)
Matrix (-M) : BLOSUM45BLOSUM50BLOSUM62BLOSUM80BLOSUM90PAM30PAM70PAM250 [see NCBI help]
Expectation value threshold for inclusion in multipass model(-h, real) : 0.0010.0020.010.1
Maximum number of passes to use in multipass version (-j, int) : 1234567 (limited to 7)